鶏コクシジウムの基礎生物学的研究 : Eimeria tenella,E.brunetti および E.acervulina の孵化鶏卵培養

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  • BASIC BIOLOGICAL STUDIES ON AVIAN COCCIDIUM DEVELOPMENT OF EIMERIA TENELLA, E.BRUNETTI AND E.ACERVULINA IN CHICK EMBRYOS
  • ケイ コクシジウム ノ キソ セイブツガクテキ ケンキュウ Eimeria tenella , E.brunetti オヨビ E.acervulina ノ フカ ケイラン バイヨウ

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Studies were made on the basic biology of coccidia, including the cultivation of repre-sentative species of avian coccidia. It was their purposes to supplement information onthe growth of Eimeria tenella, E. brunetti, and E. acervulina in avian embryos, and toexamine the biological aspects of tlaese parasites.Sporozoites and merozoites (E. tenella) were inoculated into the allantoic cavities ofchick embryos on the 1Otla day of incubation. Histological sections and unstained speci-mens of the chorioallantoic membrane (CAM) were prepared froun embryos which hadbeen killed serially at different intervals of time or died after inoculation. They wereexamined for growth of the parasites.Sporozoites were obtained by the in vitro method described by DORAN and FARR(1962). Merozoites were collected from the cecal contents of infected chickens.The results obtained are summarized as follows:1. E. tenella.a. All the inoculated embryos produced infections. A large number of schizontsdeveloped in the tissue of CAM 3s6 days after inoculation. They disappeared 7sS daysafter inoculation. At this time, the sexual stage of the parasite was observed.b. Oocysts were first seen on the 8th day (lxlO5 sporozoites per embryo) and onthe 10th day (5 x 10 sporozoites per embryo) after inoculation. The number of oocystsformed in the tissue of CAM was small, as compared with the large number of schizontsgrown.c. Many blastophore types and budding forms were seen in the CAM 5=6 daysafter inoculation with 13105 sporozoites.d. First-generation schizonts and parasites of the sexual stage were situated in theepithelial cells of CAM. Second-generation schizonts were situated below the epithelium.e. Many parasites of the sexual stage were found 2 days after inoculation withl x 106 merozoites, but no oocysts were produced by this time. When 5 x 106 merozoi teswere introduced, oocysts were recovered from the CAM 5 days after inoculation. Noparasites of the asexual stage were found in any inoculated embryo.2. Oocysts were recovered from the CAM 9 days

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