乳用種雄牛の血清フェリチン量 : ラジオイムノアッセイ法およびエンザイムイムノアッセイ法による測定

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  • Serum ferritin concentration of dairy bulls: Determinations by radioimmunoassay and enzyme immunoassay.
  • 乳用種雄牛の血清フェリチン量-ラジオイムノアッセイ法およびエンザイムイムノアッセイ法による測定〔英文〕
  • ニュウヨウ シュユウギュウ ノ ケッセイ フェリチンリョウ ラジオイムノアッセ

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Serum ferritin concentration and other iron-related blood characteristics of Holstein dairy bulls were studied. Serum ferritin concentration was measured by a two-site immunoradiometric assay (2-site IRMA). The mean value ±standard error of this protein in blood serum of dairy bulls reared in breeding stations near to Sapporo was 36±7 ng/ml in adult (n=109) and 13±1 ng/ml in young bulls (n=19), respectively. Similar changes with age were observed in mean corpuscular volume, mean corpuscular hemoglobin, hemoglobin concentration, hematocrit, total serum protein and transferrin concentration. On the other hand, count of red blood cells in young bulls was higher than that in adult ones. But sexual difference in serum ferritin concentrations was not detected between bulls and cows previously reported. Furthermore, the ferritin levels in adult bulls were also measured by an enzyme immunoassay (EIA) used horseradish peroxidase as an enzyme and ABTS as a substrate. A correlation coefficient between ferritin concentrations determined by two methods was a significant high value (r=0.990). However, in the present EIA of serum ferritin, determination of low ferritin level below 5 ng/ml was likely difficult. But this assay system may be useful to determine high serum levels of ferritin in some metabolic disorders of animals.

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