書誌事項
- タイトル別名
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- Determination of blood volume in dogs using an enriched stable isotope 50Cr.
- 安定濃縮同位体50Crを用いた犬の血液量の測定〔英文〕
- アンテイ ノウシュク ドウイタイ 50Cr オ モチイタ イヌ ノ ケツエキリ
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抄録
For the measurement of canine blood volume, various experimental conditions and techniques have been investigated using a non radioactive stable isotope 50Cr. On the basis of the results in this preliminary work, erythrocytes were labeled using 50Cr. Five μg of 50Cr per 1ml of blood was added and incubated for 60 min. The canine erythrocytes were tagged using 50Cr and injected into vein of the same dogs. The blood samples collected at 60 min after the injection were irradiated by thermal neutron for 20 min at the reactor of the JAERI. Activities of 51Cr (the 50Cr concentration method) and 51Cr/59Fe radioactivity ratios (the 51Cr/59Fe ratio method) in the samples were measured. There was a very high correlation (r=0.97, P<0.001) between the blood volumes calculated by the 50Cr concentration method and the 51Cr/59Fe ratio method. The latter method is less complicated than the former, because measurement of the sample weight and correction of thermal neutron flux are unnecessary. The mean blood volumes calculated by the ratio method and the Evans blue method were 89.8±6.8ml/kg B.W. (mean±SD) and 98.9±10.6ml/kg, respectively, showing a significant difference between them (P<0.05). However, these values are almost in accord by correction of venous blood PCV values with factor 0.97. As a detection limit of 50Cr was approximately 0.1 ng per 1ml of blood in this system, this method has been concluded to be applicable to the measurement of the blood volume of cattle.
収録刊行物
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- 日本獸醫學雜誌(The Japanese Journal of Veterinary Science)
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日本獸醫學雜誌(The Japanese Journal of Veterinary Science) 51 (5), 855-860, 1989
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390282681400459136
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- NII論文ID
- 110003918068
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- NII書誌ID
- AN00191788
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- COI
- 1:STN:280:By%2BC3Mzgt1M%3D
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- ISSN
- 18811442
- 00215295
- http://id.crossref.org/issn/00215295
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- NDL書誌ID
- 3262184
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- PubMed
- 2607738
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可