Resistance Mechanism of Chloramphenicol in <I>Streptococcus haemolyticus, Streptococcus pneumoniae</I> and <I>Streptococcus faecalis</I>

• MIYAMURA Sadao

  Department of Bacteriology, Niigata University School of Medicine

• OCHIAI Hiroshi

  Department of Bacteriology, Niigata University School of Medicine

• NITAHARA Yoshiyuki

  Department of Bacteriology, Niigata University School of Medicine

• NAKAGAWA Yoji

  Department of Bacteriology, Niigata University School of Medicine

• TERAO Michinori

  Department of Bacteriology, Niigata University School of Medicine

抄録

The chloramphenicol resistance of Streptococcus haemolyticus, Streptococcus pneumoniae and Streptococcus faecalis isolated from clinical materials was proved to be due to an inactivating enzyme produced by these bacteria. The inactivated products of chloramphenicol were identified as 1-acetoxy, 3-acetoxy and 1, 3-diacetoxy derivatives by thin-layer chromatography and infrared spectroscopy. The responsible enzyme was thus confirmed to be chloramphenicol acetyltransferase. The enzyme was inducible. It was partially purified by ammonium sulfate precipitation, DEAE-cellulose chromatography and gel filtration on Sephadex G-150. The enzymes obtained from S. haemolyticus, S. pneumoniae and S. faecalis have been compared with the conclusion that they are identical with respect to molecular weight (approximately 75, 000-80, 000), optimum pH and heat stability.

収録刊行物

• MICROBIOLOGY and IMMUNOLOGY

  MICROBIOLOGY and IMMUNOLOGY 21 (2), 69-76, 1977

  Center For Academic Publications Japan

詳細情報

• CRID: 1571417128058078592
• NII論文ID: 130003482609
• ISSN: 03855600
• Web Site: https://jlc.jst.go.jp/DN/JALC/00280412876?from=CiNii
• 本文言語コード: en
• データソース種別:
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