Effect of Molar Ratio of Fluorescent Dye to IgG on the Detection of Lymphocyte Surface Immunoglobulins by Immunofluorescence

• MURATA Michisato

  Department of Immunology, Institute of Medical Science, University of Tokyo

• KAWAMURA Jr. Akiyoshi

  Department of Immunology, Institute of Medical Science, University of Tokyo

抄録

Labeled antibodies with different F/P molar ratios of FITC to protein (F/P molar ratio) were used for the detection of surface immunoglobulin (S-Ig) of human and mouse lymphocytes by membrane immunofluorescence, and the following results were obtained.<BR>1. The percentage of S-Ig bearing cells increased markedly when labeled antihuman H- or L-chains antibodies were used with higher F/P molar ratios. The investigation of frozen kidney sections of mice injected with human immunoglobulin revealed that such an increase of the positive ratio in S-Ig was caused by increased non-specific adsorption of the fraction of labeled antibody with a high F/P molar ratio.<BR>2. This non-specific adsorption phenomenon was observed at various intensities in materials from different species; materials from mice showed less non-specific adsorption than those from humans.<BR>3. It was possible to exclude reactivity with an Fe receptor using the top one third of the supernatant of labeled antibody centrifuged at 150, 000 for 30 min.

収録刊行物

• MICROBIOLOGY and IMMUNOLOGY

  MICROBIOLOGY and IMMUNOLOGY 24 (1), 65-78, 1980

  Center For Academic Publications Japan

詳細情報

• CRID: 1570009753175109120
• NII論文ID: 130003482868
• ISSN: 03855600
• Web Site: https://jlc.jst.go.jp/DN/JALC/00188443828?from=CiNii
• 本文言語コード: en
• データソース種別:
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