Establishment of Autoantibody-Producing Cell Lines from Peripheral Blood Lymphocytes of Patients with Systemic Lupus Erythematosus

J-STAGE
  • HIRANO Toshio
    Department of Internal Medicine, Osaka Prefectural Habikino Hospital
  • TERAOKA Osamu
    Department of Internal Medicine, Osaka Prefectural Habikino Hospital
  • TERANISHI Tsuyoshi
    Department of Immunobiochemistry. Kumamoto University Medical School
  • TSUYUGUCHI Izuo
    Department of Internal Medicine, Osaka Prefectural Habikino Hospital
  • TOHDA Hiroko
    Department of Internal Medicine, Osaka Prefectural Habikino Hospital
  • OIKAWA Atsushi
    Department of Internal Medicine, Osaka Prefectural Habikino Hospital

Abstract

Autoantibody-producing B cell lines were established from peripheral blood lymphocytes of patients with systemic lupus erythematosus. Peripheral blood lymphocytes obtained from five of seven patients were successfully transformed by Epstein-Barr virus. Two of four established B lymphoblastoid cell lines examined in this study produced anti-nuclear factor antibodies and one of them produced anti-single-stranded DNA and anti-double-stranded DNA antibodies. These results indicate that B cell clones committed to self antigens are transformed by Epstein-Barr virus and continue to produce autoantibodies. In order to establish a monoclonal auto-antibody-producing B cell line, the cells were cloned by a limiting dilution method. The data suggest that it is possible to establish a monoclonal autoantibody-producing B cell line by the combination of transformation of B cells by Epstein-Barr virus and extensive cloning.

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