Ultrastructures of Leuco-Adsorption on Monolayers Infected with Influenza Virus

J-STAGE
  • SABURI Yasuo
    Department of Virology, Okayama University Medical School
  • UNO Fumio
    Department of Virology, Okayama University Medical School
  • AKATSUKA Kazuya
    Department of Virology, Okayama University Medical School
  • UNO Naomichi
    Department of Virology, Okayama University Medical School
  • KUMON Hiromi
    Department of Virology, Okayama University Medical School
  • TAKAHASHI Toyozoh
    Department of Bacteriology, Yokohama City University School of Medicine
  • OKUDA Kenji
    Department of Bacteriology, Yokohama City University School of Medicine

Abstract

Leuco-adsorption occurring in influenza virus infected-cell cultures was studied morphologically to clarify the mechanisms of adsorption of leukocytes. Among the various types of chicken leukocytes studied, such as lymphocytes, monocytes, granulocytes, and thrombocytes, all were found to adhere to the virus-infected cells. The adsorption seems to occur through at least two processes, one is mediated by microvilli (microvillus-attachment), and the other is direct adherence of both cells (cell-to-cell-attachment). In the former, the leukocytes are bound to the microvilli protruding from the infected MDCK cells and in the latter both cell membranes attach directly. In the cell-to-cell-attachment, there was an electron-lucent gap of about 12nm in width in the intermembranous space of the junctional regions. This region was similar morphologically to the gap junction. As a result of leuco-adsorption no cytolytic effects occurred in the MDCK cells under the experimental conditions.

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