Synthesis and Deposition of Spore Coat Proteins during Sporulation of <i>Bacillus megaterium</i>
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- IMAGAWA Masayoshi
- Department of Environmental Chemistry and Microbiology, Faculty of Pharmaceutical Sciences, Osaka University
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- OKU Yuichi
- Department of Environmental Chemistry and Microbiology, Faculty of Pharmaceutical Sciences, Osaka University
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- EL-BELBASI Hussein I.
- Department of Environmental Chemistry and Microbiology, Faculty of Pharmaceutical Sciences, Osaka University
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- TERAOKA Mie
- Department of Environmental Chemistry and Microbiology, Faculty of Pharmaceutical Sciences, Osaka University
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- NISHIHARA Tsutomu
- Department of Environmental Chemistry and Microbiology, Faculty of Pharmaceutical Sciences, Osaka University
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- KONDO Masaomi
- Department of Environmental Chemistry and Microbiology, Faculty of Pharmaceutical Sciences, Osaka University
抄録
Rabbit (anti-spore coat protein) IgG was prepared by immunization with coat proteins extracted with sodium dodecyl sulfate and dithiothreitol from isolated spore coats of Bacillus megaterium ATCC 12872. Coat proteins were detected from 3hr after the end of exponential growth (t3) in the mother cell cytoplasmic fraction by sandwich enzyme immunoassay using this antibody. The proteins in the forespore coat protein fraction increased from t3 and reached a plateau at t10, Immunoblot analysis for the coat proteins in sporulating cells revealed the sequential synthesis of various proteins in the mother cell cytoplasmic fraction and simultaneous deposition of the same proteins as in the forespore coat fraction. These results suggest that turnover of precursor proteins of the spore coat is very rapid if precursor proteins are produced and they are proteolytically processed to produce mature proteins. Specific antibody to the 48, 000-dalton protein, which is a major protein, did not cross-react with any other major (36, 000, 22, 000, 19, 500, and 17, 500-dalton) proteins.
収録刊行物
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- MICROBIOLOGY and IMMUNOLOGY
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MICROBIOLOGY and IMMUNOLOGY 29 (12), 1151-1162, 1985
Center For Academic Publications Japan
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詳細情報 詳細情報について
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- CRID
- 1573387452895122560
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- NII論文ID
- 130003483085
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- ISSN
- 03855600
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles