A Staphylococcal Coagglutination Test for Detecting and Serogrouping <i>Legionella pneumophila</i>

J-STAGE
  • YOH Myonsun
    Research Institute for Microbial Diseases, Osaka University
  • HONDA Takeshi
    Research Institute for Microbial Diseases, Osaka University
  • MIWATANI Toshio
    Research Institute for Microbial Diseases, Osaka University
  • UEMURA Takaaki
    The Research Foundation for Microbial Diseases of Osaka University
  • KUWATA Hiroyuki
    The Research Foundation for Microbial Diseases of Osaka University
  • FUKAI Konosuke
    The Research Foundation for Microbial Diseases of Osaka University

抄録

For detection of Legionella pneumophila and determination of its serogroup, the modified staphylococcal coagglutination test was studied in detail. Crossreactions in serum-agglutination tests were observed among serogroups, but immuno-globulin-coated staphylococcal cells could detect L. pneumophila with high sensitivity (a cell concentration with an absorbance of 0.008 at 660nm could be detected) and serogroups could be determined without cross-reactions. Moreover the coexistence of other bacteria did not affect the results of the test. These results suggest that the staphylococcal coagglutination test is useful for detection and identification of Legionella, especially in environmental samples, and for serogrouping of isolates of L. pneumophila from clinical specimens.

収録刊行物

詳細情報 詳細情報について

問題の指摘

ページトップへ