The Suitability of Tórtora's Medium for the Production of Enterotoxin in <i>Clostridium perfringens</i> Strains
-
- TÓRTORA João Carlos DE OLIVEIRA
- Instituto Gonzaga da Gama Filho, Universidade Gama Filho
-
- COSTA Gobert Araujo
- Instituto Gonzaga da Gama Filho, Universidade Gama Filho
抄録
Examination of 200 samples from soil and the same number of samples from healthy human feces yielded 49 (24.5%) and 105 (52.5%) strains of heat-resistant Clostridium perfringens respectively. Fourteen (7.0%) strains isolated from soil and 37 (18.5%) from feces synthesized enterotoxin, as demonstrated by Tórtora's method, at sufficient levels to permit its detection by mouse lethality, microslide double gel diffusion or counterimmunoelectrophoresis tests, By using the Duncan-Strong (DS) method, only four (2%) enterotoxigenic strains from soil and 14 (7.0%) from feces were obtained. The supernatant fluid from two enterotoxigenic-negative strains grown in DS medium gave a false-positive reaction when they were injected intravenously into mice. Tórtora's medium was preferable because a larger number of isolated strains produced spores and enterotoxin to permit their recognition as enterotoxigenic strains.
収録刊行物
-
- MICROBIOLOGY and IMMUNOLOGY
-
MICROBIOLOGY and IMMUNOLOGY 29 (6), 509-516, 1985
Center For Academic Publications Japan
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1573387452894601856
-
- NII論文ID
- 130003483147
-
- ISSN
- 03855600
-
- 本文言語コード
- en
-
- データソース種別
-
- CiNii Articles