Structural Changes in the Nucleoid of <i>Bacillus subtilis</i> at Low Temperature

• AMAKO Kazunobu

  Department of Bacteriology, Faculty of Medicine, Kyushu University

• TAKADE Akemi

  Department of Bacteriology, Faculty of Medicine, Kyushu University

• UMEDA Akiko

  Department of Bacteriology, Faculty of Medicine, Kyushu University

抄録

The external shape of the nucleoid of Bacillus subtilis strain w23 was examined with a new electron microscopic technique, the rapid freezing and substitution fixation method. The nucleoid of the log and stationary phase cells was recognized as an area devoid of ribosomes and widely dispersed in the cytoplasm, which was different from that observed in OsO₄-fixed cells. If the bacteria were exposed to low temperatures (0 to 10C), the nucleoid showed a highly concentrated shape in the middle of the cytoplasm. These structural changes were observed only when the bacteria were maintained in a high-salt buffer. The results are discussed in relation to the membrane fluidity at low temperature.

収録刊行物

• MICROBIOLOGY and IMMUNOLOGY

  MICROBIOLOGY and IMMUNOLOGY 30 (9), 855-861, 1986

  Center For Academic Publications Japan

詳細情報

• CRID: 1570009753174481536
• NII論文ID: 130003483262
• ISSN: 03855600
• Web Site: https://jlc.jst.go.jp/DN/JALC/00171412190?from=CiNii
• 本文言語コード: en
• データソース種別:
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