Purification of a κ-Carrageenase from Marine <i>Cytophaga</i> Species

• SARWAR Golam

  Department of Bacteriology, Faculty of Medicine, Kagoshima University

• MATAYOSHI Seiken

  Department of Bacteriology, Faculty of Medicine, Kagoshima University

• ODA Hiroshi

  Department of Bacteriology, Faculty of Medicine, Kagoshima University

抄録

A mixture of extracellular carrageenases was isolated from the cell-free medium of a culture of marine Cytophaga sp. 1k-C783 grown on ZoBell 2216 E broth with 0.1% commercial carrageenan. A single active peak of κ-carrageenase was separated and purified from the mixture by ammonium sulfate precipitation, ion-exchange chromatography, and Sephadex G-200 gel filtration chromatography. Molecular weight of the purified κ-carrageenase was estimated as 100, 000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The purified κ-carrageenase had pH optimum 7.6 and temperature optimum 25C.

収録刊行物

• MICROBIOLOGY and IMMUNOLOGY

  MICROBIOLOGY and IMMUNOLOGY 31 (9), 869-877, 1987

  Center For Academic Publications Japan

詳細情報

• CRID: 1572543027964665088
• NII論文ID: 130003483382
• ISSN: 03855600
• Web Site: https://jlc.jst.go.jp/DN/JALC/00172238034?from=CiNii
• 本文言語コード: en
• データソース種別:
  • CiNii Articles