Antigenic Determinants on Fimbriae of <i>Serratia marcescens</i> US5 Analyzed Using Monoclonal Antibodies
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- JINGUSHI Seiya
- Department of Bacteriology, Faculty of Medicine, Kyushu University
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- MITSUYAMA Masao
- Department of Bacteriology, Faculty of Medicine, Kyushu University
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- MORIYA Tetsuhiro
- Department of Bacteriology, Faculty of Medicine, Kyushu University
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- KAWABATA Shunichiro
- Department of Biology, Faculty of Science, Kyushu University
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- IWANAGA Sadaaki
- Department of Biology, Faculty of Science, Kyushu University
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- AMAKO Kazunobu
- Department of Bacteriology, Faculty of Medicine, Kyushu University
抄録
The antigenic sites on small thin fimbriae of Serratia marcescens strain US5 were investigated using immunoelectron microscopy and monoclonal antibodies (MAbs). Negative staining of the fimbriae after treatment with MAbs showed a regularly spaced arrangement of the antibody molecules. When the subunit peptide was subjected to immunoblotting using the MAbs, a single band with a molecular weight of approximately 19kD was evident. This binding of the MAbs to the subunit peptide was completely abrogated after treatment with 2-mercaptoethanol, thereby suggesting the important role of disulfide linkage in the maintenance of the conformation of the antigenic site reacted with MAbs. Amino acid analysis of the subunit peptide revealed two cysteine residues, and cysteine residues were absent in the N-terminal portion.
収録刊行物
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- MICROBIOLOGY and IMMUNOLOGY
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MICROBIOLOGY and IMMUNOLOGY 31 (9), 879-889, 1987
Center For Academic Publications Japan
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詳細情報
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- CRID
- 1570572703127708288
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- NII論文ID
- 130003483388
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- ISSN
- 03855600
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles