Role of a Guanine Nucleotide-Binding Regulatory Protein in the Hydrolysis of Phosphatidylinositol 4, 5-Bisphosphate in a Human T Cell Line
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- HASEGAWA-SASAKI Hiroko
- Department of Biochemistry, Sapporo Medical College
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- LUTZ Frieder
- Institute for Pharmacology and Toxicology, Justus-Liebig University
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- SASAKI Terukatsu
- Department of Biochemistry, Sapporo Medical College
抄録
The CD3(T3)/antigen receptor complex appears to function by transducing an antigen signal presented by macrophages into the hydrolysis of phosphatidylinositol 4, 5-bisphosphate [PtdIns(4, 5)P2]. In order to find out how the CD3/antigen receptor complex regulates the hydrolysis of Ptdlns(4, 5)P2 to diacylglycerol and inositol trisphosphate, we investigated the possible role played by a guanine nucleotide-binding regulatory protein in PtdIns(4, 5)P2 hydrolysis in a human T cell leukemia line, JURKAT. JURKAT cells were made permeable to Al3+, F-, GTP, and a nonhydrolyzable GTP analogue, guanosine 5'-O-(3-thiotriphosphate) (GTPγS), by treatment with pseudomonal cytotoxin. In the presence of AlCl3 NaF stimulated the release of inositol phosphates in the cytotoxin-treated JURKAT cells. NaF plus AlCl3 induced increases in inositol tris-, bis-, and mono-phosphates and decreases in PtdIns(4, 5)P2, phosphatidylinositol 4-phosphate, and phosphatidylinositol within 5min after addition to the cytotoxin-treated cells at 37C. GTPγS stimulated, to some extent, polyphosphoinositide hydrolysis in the cytotoxin-treated JURKAT. The cytotoxin-treated JURKAT cells retained the ability to respond to anti-Leu-4 with polyphosphoinositide hydrolysis. It has been shown that Al3+ in the presence of F- modulates the activity of various guanine nucleotide-binding regulatory proteins. Therefore, the results obtained in this study indicate that a guanine nucleotide-binding regulatory protein regulates the polyphosphoinositide breakdown in JURKAT cells by influencing phosphodiesterase activity.
収録刊行物
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- MICROBIOLOGY and IMMUNOLOGY
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MICROBIOLOGY and IMMUNOLOGY 32 (3), 293-304, 1988
Center For Academic Publications Japan
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詳細情報 詳細情報について
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- CRID
- 1572543027965393152
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- NII論文ID
- 130003483437
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- ISSN
- 03855600
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles