Possibility of <i>Bacteroides gingivalis</i> Hemagglutinin Possessing Protease Activity Revealed by Inhibition Studies

• NISHIKATA Makoto

  Central Research Division, School of Dentistry, Hokkaido University

• YOSHIMURA Fuminobu

  Department of Microbiology, School of Dentistry, Hokkaido University

• NODASAKA Yoshinobu

  Central Research Division, School of Dentistry, Hokkaido University

抄録

Inhibition of hemagglutinin (HA) activity in a membrane fraction of Bacteroides gingivalis was examined using various compounds. Leupeptin and antipain inhibited the HA activity at nM order. This potency was lost when the aldehyde group of leupeptin was converted to an alcohol moiety. Irreversible protease inhibitors, tosyl-L-lysine chloromethyl ketone (TLCK), p-chloromercuribenzoate (PCMB), and N-ethylmaleimide (NEM) were also inhibitory. From the inhibition experiments, we speculate that the HA possesses protease activity and that the same site of the molecule participates in the erythrocyte binding and the substrate binding.

収録刊行物

• MICROBIOLOGY and IMMUNOLOGY

  MICROBIOLOGY and IMMUNOLOGY 33 (1), 75-80, 1989

  Center For Academic Publications Japan

詳細情報

• CRID: 1570009753175127168
• NII論文ID: 130003483518
• ISSN: 03855600
• Web Site: https://jlc.jst.go.jp/DN/JALC/00173513918?from=CiNii
• 本文言語コード: en
• データソース種別:
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