A Simple Method for Observation of Phagocytosis on Bacterial Thin-Layer

J-STAGE
  • SEKI Keiko
    Department of Bacteriology, The Jikei University School of Medicine
  • MURAI Miyo
    Department of Bacteriology, The Jikei University School of Medicine
  • SAKURADA Junji
    Department of Bacteriology, The Jikei University School of Medicine
  • SHIRAHIGE Akira
    Department of Orthopaedics, The Jikei University School of Medicine
  • KOBAYASHI Noriyuki
    Department of Periodontology, Nippon Dental University
  • HWANG Soo-Myung
    Department of Clinical Pathology, Jisan Junior College
  • MASUDA Shogo
    Department of Bacteriology, The Jikei University School of Medicine

抄録

A simple method was developed to visually present the phagocytic activity of leukocytes by using adherent Staphylococcus aureus cells and blood applied on a plastic dish. When heparinized blood was applied on thin-layer of heat-killed S. aureus cells on the plastic dish, plaques due to the phagocytic activity of leukocytes were observed with a microscope under a low magnification. Fewer and smaller plaques were observed when plasma-deprived rather than whole blood was used. Some analyses were made in respect to the fundamental conditions required for optimal results. This method was considered to be useful for conveniently evaluating the serum opsonin activities and phagocytic function of leukocytes in various kinds of diseases.

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