A Novel Neutrophil Adherence Test Effectively Reflects the Activated State of Neutrophils

J-STAGE
  • YAKUWA Naoshi
    The Department of Parasitology, Yamagata University School of Medicine
  • INOUE Tomio
    The Department of Parasitology, Yamagata University School of Medicine
  • WATANABE Tadashi
    The Department of Parasitology, Yamagata University School of Medicine
  • TAKAHASHI Keiji
    The First Department of Internal Medicine, Yamagata University School of Medicine
  • SENDO Fujiro
    The Department of Parasitology, Yamagata University School of Medicine

抄録

We developed a novel and convenient neutrophil adherence test for ascertaining the activated state of neutrophils. Human peripheral blood neutrophils were placed in wells of a 96-well flat-bottom culture plate, and incubated in the presence or absence of neutrophil stimulants for varying periods of time at 37C in a CO2 incubator (5% CO2, 95% air). After non-adherent cells were completely removed by vibration on a vortex mixer, residual adherent cells were fixed and stained with crystal violet containing 12% formaldehyde and 10% ethanol. After thorough washing, 1% SDS was added to the plate, and the absorbance of each well was measured at 570nm. Our results correlate well with those of a previously reported method using 51Cr-labeled cells.

収録刊行物

詳細情報 詳細情報について

問題の指摘

ページトップへ