Electron Microscopic Observations on Ciliated Epithelium of Tracheal Organ Cultures Infected with <i>Bordetella bronchiseptica</i>

• SEKIYA Kachiko

  Department of Microbiology, School of Pharmaceutical Sciences, Kitasato University

• FUTAESAKU Yutaka

  Department of Ultrastructure Research, School of Hygienic Sciences, Kitasato University

• NAKASE Yasukiyo

  Department of Microbiology, School of Pharmaceutical Sciences, Kitasato University

抄録

Using mouse tracheal organ cultures, the pathogenic effect of Bordetella bronchiseptica to epithelial cells was studied by electron microscopy. The ultrastructure of epithelial cells in uninfected tracheal rings was preserved well for longer than 3 days. In mouse tracheal rings infected with graded doses (3×10⁵ to 10⁷ CFU/ml) of phase I B. bronchiseptica, the colonization in the interciliary spaces of ciliated epithelial cells was observed after a 20-hr infection period. The infected tracheal rings showed swelling of nonciliated cells as well as ciliated cells, rupture of cell membrane of cilia, swelling and disappearance of cilia, and atrophic cytomorphosis of epithelial cells. The severity of these changes occurred depending on the infection doses. These changes were essentially similar to those observed previously in the tracheal epithelia of the B. bronchiseptica-infected mice. The usefulness of this in vitro model was suggested for studying the pathogenesis of Bordetella infection.

収録刊行物

• MICROBIOLOGY and IMMUNOLOGY

  MICROBIOLOGY and IMMUNOLOGY 33 (2), 111-121, 1989

  Center For Academic Publications Japan

詳細情報

• CRID: 1572824502942234496
• NII論文ID: 130003483553
• ISSN: 03855600
• Web Site: https://jlc.jst.go.jp/DN/JALC/00173551767?from=CiNii
• 本文言語コード: en
• データソース種別:
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