Bindings of Toxic Shock Syndrome Toxin-1 and Staphylococcal Enterotoxins A, B, and C to Rabbit Spleen Cells

• FUJIKAWA Hiroshi

  Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health

• TAKAYAMA Hiroo

  Department of Infectious Disease, Yakult Central Institute for Microbiological Research

• UCHIYAMA Takehiko

  Department of Microbiology, Tokyo Women's Medical College

• IGARASHI Hideo

  Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health

抄録

Toxic shock syndrome toxin-1 (TSST-1) and staphylococcal enterotoxins (SE) A, B, and C were studied on binding to rabbit spleen cells. The toxins showed remarkable mitogenic effects on the cells. Among them, SEA and TSST-1 had much stronger mitogenic activities than SEB and SEC. Binding study showed that labeled TSST-1 and SEA bound considerably to cells, but that labeled SEB or SEC was not observed to bind at a detectable level under the same conditions as TSST-1 and SEA. Competitive binding analysis between toxins to cells proved that TSST-1 and SEA clearly competed with each other in binding. Scatchard plots for TSST-1 and SEA in binding were linear at the doses used. The Scatchard analysis for TSST-1 and SEA gave a dissociation constant of 2.5×10^-9M and 7.6× 10^-8M and the number of binding sites per cell of 5.3×10³ and 1.0×10⁵, respectively.

収録刊行物

• MICROBIOLOGY and IMMUNOLOGY

  MICROBIOLOGY and IMMUNOLOGY 33 (5), 381-390, 1989

  Center For Academic Publications Japan

詳細情報

• CRID: 1571417128058668928
• NII論文ID: 130003483568
• ISSN: 03855600
• Web Site: https://jlc.jst.go.jp/DN/JALC/00173765287?from=CiNii
• 本文言語コード: en
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