Granuloma-Forming Activity and Antitumor Activity of Newly Isolated Mycoloyl Glycolipid from <i>Rhodococcus terrae</i> 70012 (Rt. GM-2)
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- NATSUHARA Yayoi
- Department of Bacteriology, Osaka City University Medical School
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- YOSHINAGA Junji
- Osaka Research Institute, Sawai Pharmaceutical Co., Ltd.
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- SHOGAKI Takeshi
- Osaka Research Institute, Sawai Pharmaceutical Co., Ltd.
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- SUMI-NISHIKAWA Yukie
- Osaka Research Institute, Sawai Pharmaceutical Co., Ltd.
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- KURANO Satoko
- Osaka Research Institute, Sawai Pharmaceutical Co., Ltd.
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- KATO Yoshiko
- Osaka Research Institute, Sawai Pharmaceutical Co., Ltd.
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- KANEDA Kenji
- Department of Anatomy, Faculty of Medicine, Tokyo Medical and Dental University
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- OKA Shiro
- Department of Bacteriology, Osaka City University Medical School
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- YANO Ikuya
- Department of Bacteriology, Osaka City University Medical School
抄録
A newly isolated mycoloyl glycolipid (Rt. GM-2) from Rhodococcus terrae 70012 was identified and the granulomagenic and antitumor activities were studied as compared with trehalose-6, 6'-dimycolate (cord factor) also from R. terrae (Rt. TDM). The alkaline hydrolysis products of Rt. GM-2 contained trehalose, methyl-α-mycolate and a less-polar ester than the usual methyl-α-mycolate, possibly β-keto mycolate (1:1:1, by mol. ratios). On the other hand, analysis of alditol acetate obtained after the mild permethylation, NaBH4 reduction, and acetylation showed the occurrence of 2, 3, 4-tri-O-methyl-6-O-acetylglucitol. Therefore, the original glycolipid (Rt. GM-2) was identified tentatively as 6-O-α-mycoloyl 6'-O-β-ketomycoloyl trehalose. Intravenous injection of Rt. GM-2 in the form of water-in-oil-in-water emulsion caused prominent granulomas in lungs and spleen of ICR and BALB/c mice. The granulomagenic effects were as strong as those caused by Rt. TDM. The lung and spleen weights reached peaks one week after an injection of Rt. GM-2 in mice and then gradually decreased. Multiple intravenous injections of Rt. GM-2 and Rt. TDM showed antitumor activity against subcutaneously implanted Sarcoma-180, and caused prominent granulomatous changes and growthsuppression of mice.
収録刊行物
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- MICROBIOLOGY and IMMUNOLOGY
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MICROBIOLOGY and IMMUNOLOGY 34 (1), 45-53, 1990
Center For Academic Publications Japan
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詳細情報 詳細情報について
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- CRID
- 1571698603035247872
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- NII論文ID
- 130003483617
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- ISSN
- 03855600
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles