Diversity of Matrix Protein in Subacute Sclerosing Panencephalitis and Measles Virus-Infected Cells

J-STAGE
  • HAGA Takeshi
    Laboratory Animal Research Center, Institute of Medical Science, The University of Tokyo
  • YOSHIKAWA Yasuhiro
    Department of Animal Pathology, Institute of Medical Science, The University of Tokyo
  • YAMANOUCHI Kazuya
    Laboratory Animal Research Center, Institute of Medical Science, The University of Tokyo

Abstract

Expression of the viral matrix (M) proteins in Vero cells infected with 18 strains of subacute sclerosing panencephalitis (SSPE) virus and measles virus was examined by immunocytochemistry and Western blot analysis using an anti-M monospecific serum and two sera against the M protein specific synthetic peptides. By immunocytochemistry using the anti-M monospecific serum, M protein was detected in all of the virus-infected cells regardless of cell-free virus production. M proteins of the seven non-productive strains were found to vary significantly in their epitope, in their reactivity to different assay systems, and in their molecular weight, whereas M proteins of the other 11 productive strains were detected consistently. These results suggest diversification of M protein of the non-productive strains.

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