Japanese Encephalitis Virus Fusion Protein with Protein A Expressed in <i>Escherichia coli</i> Confers Protective Immunity in Mice

J-STAGE
  • SRIVASTAVA Ashok Kumar
    Department of Virology, Institute of Tropical Medicine, Nagasaki University
  • MORITA Kouichi
    Department of Virology, Institute of Tropical Medicine, Nagasaki University
  • MATSUO Sachiko
    Department of Virology, Institute of Tropical Medicine, Nagasaki University
  • TANAKA Mariko
    Department of Virology, Institute of Tropical Medicine, Nagasaki University
  • IGARASHI Akira
    Department of Virology, Institute of Tropical Medicine, Nagasaki University

抄録

A complementary DNA (cDNA) that codes C-terminal, one-third of envelope glycoprotein (E) and N-terminal 65 amino acids of NS1 protein of Japanese encephalitis (JE) virus was inserted into Escherichia coli expression vector pRIT2T. The inserted gene was expressed as a fusion protein with protein A, and the expressed protein was intraperitoneally injected into mice. The immunized mice produced anti-JE antibodies measured by the hemagglutination-inhibition and neutralization tests as well as ELISA and were protected from the lethal challenge of JE virus by intraperitoneal inoculation.

収録刊行物

詳細情報 詳細情報について

問題の指摘

ページトップへ