New Enzyme Immunoassays for Specific Assay and General Detection of <i>Trypanosoma cruzi</i>, Epimastigotes
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- KITAGAWA Tsunehiro
- Faculty of Pharmaceutical Sciences, Institute of Tropical Medicine, Nagasaki University
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- IWAMOTO Masayuki
- Faculty of Pharmaceutical Sciences, Institute of Tropical Medicine, Nagasaki University
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- ZHAO Li-Ping
- Faculty of Pharmaceutical Sciences, Institute of Tropical Medicine, Nagasaki University
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- KANBARA Hiroji
- Department of Protozoology, Institute of Tropical Medicine, Nagasaki University
抄録
A highly specific competitive enzyme-linked immunosorbent assay for the epimastigote of Tulahuen strain was developed by using the usual 3 immunological reagents, a rabbit antiserum specific for T. cruzi, epimastigote of Tulahuen strain, β-D-galactosidase-labeled goat anti-rabbit immunoglobulin G and the solid-phase cell fragments of the epimastigote of Tulahuen strain. A new method, the selected antibody enzyme immunoassay (SAEIA) which generally detected all strains of the epimastigote tested with the same working range, was developed by changing only the solid-phase antigen to the epimastigote of Y strain among the 3 immunological reagents. Both assays permitted us to measure accurately as little as 1, 000 parasites per assay tube. Scope of the SAEIA was limited to the epimastigote. Both lifecycle forms of T. cruzi which appear in mammals, amastigote and trypomastigote, and other kinetoplastids showed low cross-reaction values by the assay. The assay principle of the new method and a preliminary study to apply the SAEIA for findingthe field T. cruzi-infected insect vectors were also reported.
収録刊行物
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- MICROBIOLOGY and IMMUNOLOGY
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MICROBIOLOGY and IMMUNOLOGY 35 (11), 943-951, 1991
Center For Academic Publications Japan
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詳細情報 詳細情報について
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- CRID
- 1571980078011122816
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- NII論文ID
- 130003483725
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- ISSN
- 03855600
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles