Two Generalized Transducing Phages in <i>Vibrio parahaemolyticus</i> and <i>Vibrio alginolyticus</i>
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- MURAMATSU Kouichi
- Department of Infectious Disease, Nagano Research Institute for Health and Pollution
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- MATSUMOTO Hideki
- Department of Bacteriology, School of Medicine, Shinshu University
抄録
Two bacteriophages named φVP253 and φVP143 isolated after ultraviolet induction from lysogenic strains of Vibrio parahaemolyticus have been shown to be generalized transducing phages. So far, seven different auxotrophic markers of a V. parahaemolyticus strain could be transduced at the frequencies ranging from 2.2× 10-7 to 7.5×10-5 per infected cell at the m.o.i. of approximately 1.0. The phage φVP143, but not φVP253, lysed 20 of the 28 strains of V. alginolyticus and the occurrence of generalized transduction by this phage in this Vibrio species has been confirmed. Molecular size of the genomes of both phages were estimated to be approximately 48kb as judged from electrophoretic mobilities of the DNAs digested with HindIII endonuclease. The results and similarity of the two phages in morphology and other properties suggest very close relatedness of the phages.
収録刊行物
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- MICROBIOLOGY and IMMUNOLOGY
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MICROBIOLOGY and IMMUNOLOGY 35 (12), 1073-1084, 1991
財団法人 学会誌刊行センター