Prethymic Nylon Wool-Passed Bone Marrow Cells, Substituting for Helper T Cells, Can Augment the Generation of Cytotoxic T Lymphocytes from Their Precursors
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- TANABE Masao J.
- Department of Bacteriology, School of Medicine, University of the Ryukyus
抄録
Nylon wool-passed bone marrow (NW-BM) cells treated with anti-Thy.1 monoclonal antibody and complement were added to a mixed lymphocyte culture which contained a limiting number of lymph node cells, as responder cells, and a sufficient number of mitomycin-c-treated allogeneic spleen cells as stimulator cells. NW-BM cells of the same MHC haplotype as responder cells enhanced the generation of allo-specific cytotoxic T lymphocytes (CTL) not only at a relatively high dose (3×103 cells/well) of responder cells, but also at an extremely dilute dose (1×103 cells/well). NW-BM cells which had a third-party MHC haplotype, a haplotype different from both responder and stimulator cells, also enhanced the generation of CTL at relatively high doses, but not at low doses, of responder cells. NW-BM cells which had MHC haplotypes identical with those of responder cells induced CTL from helper T cell-depleted responder cells, but NW-BM cells which had the third-party haplotype did not. These results showed that the enhancing effects of NW-BM cells of the same MHC haplotype as responder cells might be due to a specific helper effect and the enhancing effect of NW-BM cells of the third-party haplotype might be due to a nonspecific filler effect, which only conditioned the cultured cells. It was also found that, to exhibit the helper effect, NW-BM cells had to possess MHC class II, but not MHC class I, molecules in common with CTL precursors. This study showed that in the induction of CTL, prethymic NW-BM cells had a capability comparable to that of mature helper T cells.
収録刊行物
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- MICROBIOLOGY and IMMUNOLOGY
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MICROBIOLOGY and IMMUNOLOGY 35 (12), 1115-1130, 1991
Center For Academic Publications Japan
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詳細情報 詳細情報について
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- CRID
- 1571135653080993024
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- NII論文ID
- 130003483742
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- ISSN
- 03855600
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- 本文言語コード
- en
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- データソース種別
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