Protease Inhibitors Prevent the Development of Human Rotavirus-Induced Diarrhea in Suckling Mice
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- EBINA Takusaburo
- Department of Bacteriology, Tohoku University School of Medicine
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- TSUKADA Keiko
- Department of Bacteriology, Tohoku University School of Medicine
抄録
Oral inoculation of human rotavirus MO strain (serotype 3) into 5-day-old BALB/c mice caused gastroenteritis characterized by diarrhea (90% on the average, on day 2). Using this animal model, preventive effect of antiviral agents on the development of rotavirus-induced diarrhea was examined. The infectivity of human rotavirus was enhanced by treatment with protease in vitro. A cysteine protease inhibitor, E-64-c, was given orally at 12hr and 24hr after MO infection. Oral administration of 0.3mg of E-64-c decreased the diarrhea ratio to 17.5% on day 2 and to 10% on day 3. Oral administration of 0.15mg of cysteine protease inhibitor, ovocystatin, completely prevented the diarrhea on day 2. Serine protease inhibitor, aprotinin (0.15mg×2), also prevented the diarrhea on day 2 to 14.3%. These protease inhibitors were nontoxic in vitro and to suckling mice. The histopathological changes in the small intestine due to infection recovered 2 days after MO infection in mice treated with E-64-c and ovocystatin. These results suggest that protease inhibitors are protective agents for human rotavirus infection by inhibiting proteases required for viral replication.
収録刊行物
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- MICROBIOLOGY and IMMUNOLOGY
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MICROBIOLOGY and IMMUNOLOGY 35 (7), 583-588, 1991
Center For Academic Publications Japan
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詳細情報
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- CRID
- 1573950402848112384
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- NII論文ID
- 130003483797
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- ISSN
- 03855600
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles