cDNA Cloning of an Aspartic Proteinase Secreted by <i>Candida albicans</i>

• MUKAI Hiroyuki

  Biotechnology Research Laboratories, Takara Shuzo Co., Ltd.

• TAKEDA Osamu

  Biotechnology Research Laboratories, Takara Shuzo Co., Ltd.

• ASADA Kiyozo

  Biotechnology Research Laboratories, Takara Shuzo Co., Ltd.

• KATO Ikunoshin

  Biotechnology Research Laboratories, Takara Shuzo Co., Ltd.

• MURAYAMA Somay Yamagata

  Department of Bacteriology, Teikyo University School of Medicine

• YAMAGUCHI Hideyo

  Department of Bacteriology, Teikyo University School of Medicine

抄録

cDNA of an aspartic proteinase secreted by Candida albicans No. 114 was isolated using the polymerase chain reaction (PCR). The primary structure of the enzyme was deduced from the nucleotide sequence of the cDNA and compared with the structures of Saccharomyces cerevisiae proteinase A and vacuolar aspartyl proteinase of C. albicans. The mature aspartic proteinase consisted of 341 amino acid residues, and was 17.6 and 15.3% identical with the proteinase A and the aspartyl proteinase, respectively. Two active aspartic acid sites and the amino acids near those sites were conserved in the aspartic proteinase. We also showed that there is another gene of aspartic proteinase than that of strain ATCC10231 reported by Hube et al (J. Med. Vet. Mycol. 29 (1991)) in the same C. albicans genome, both in that strain and in No. 114.

収録刊行物

• MICROBIOLOGY and IMMUNOLOGY

  MICROBIOLOGY and IMMUNOLOGY 36 (11), 1207-1216, 1992

  Center For Academic Publications Japan

詳細情報

• CRID: 1572261552987771136
• NII論文ID: 130003483842
• ISSN: 03855600
• Web Site: https://jlc.jst.go.jp/DN/JALC/00079130660?from=CiNii
• 本文言語コード: en
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