Cloning and Nucleotide Sequence Analysis of a Chloramphenicol Acetyltransferase Gene from <i>Vibrio anguillarum</i>

• ZHAO Jiang

  Department of Biological Resources, Faculty of Agriculture, Miyazaki University

• AOKI Takashi

  Department of Biological Resources, Faculty of Agriculture, Miyazaki University

抄録

The chloramphenicol resistant gene (cat) encoding chloramphenicol acetyltransferase (CAT) in a transferable R plasmid (pJA7324) isolated from the fish pathogen Vibrio anguillarum strain PT24 was cloned into the plasmid vector pUC19. The nucleotide sequence analysis of 1, 348 base pair DNA identified an open reading frame encoding a protein of 216 amino acid residues with a calculated molecular mass of 25, 471 daltons. The predicted amino acid sequences for this cat gene are 37-69% homologous with other CAT proteins of both Gram-negative and -positive bacteria. Colony hybridization performed with a PvuII-BamHI fragment including this cat gene as a probe, revealed that the same or similar chloramphenicol resistance genes existed among V. anguillarum isolates.

収録刊行物

• MICROBIOLOGY and IMMUNOLOGY

  MICROBIOLOGY and IMMUNOLOGY 36 (7), 695-705, 1992

  Center For Academic Publications Japan

詳細情報

• CRID: 1573105977917913216
• NII論文ID: 130003483897
• ISSN: 03855600
• Web Site: https://jlc.jst.go.jp/DN/JALC/00078847507?from=CiNii
• 本文言語コード: en
• データソース種別:
  • CiNii Articles