Correlative Detection of Human Immunodeficiency Virus (HIV) Antigen p24 and Epstein-Barr Virus DNA <i>In Vitro</i>: Clinical Influence on HIV Infection

J-STAGE
  • LARDELLI Pilar
    Department of Microbiology and Immunology, School of Medicine, University of the Basque Country
  • MANZANO Diego
    Department of Microbiology and Immunology, School of Medicine, University of the Basque Country
  • STEINBERG Seth M.
    Biostatistics and Data Management Section, National Cancer Institute, National Institutes of Health
  • MADARIAGA Lucila
    Department of Microbiology and Immunology, School of Medicine, University of the Basque Country
  • ANTÓN Isabel
    Department of Microbiology and Immunology, School of Medicine, University of the Basque Country
  • CISTERNA Ramón
    Department of Microbiology and Immunology, School of Medicine, University of the Basque Country

抄録

The existence of molecular transactivations between EBV and HIV-1, as well as reactivations of EBV latent infections in AIDS patients, have been recently documented. In order to shed more light on the putative association between EBV and HIV, and its role in the evolution to AIDS, we have determined simultaneously p24 protein and EBV DNA in culture supernatants of peripheral blood mononuclear cells from 47 individuals suspected of having HIV infection. The results of the in vitro assays were correlated with the clinical stage of the individuals and their serologic status to EBV. Statistical analysis showed a concordance between HIV infection and in vitro detection of EBV DNA (P<0.002); particularly, a strong correlation between the presence of EBV DNA and p24 in culture was observed (P<0.001). These results are consistent with the occurrence of viral interactions, manifested in vitro. However, in our series, the appearance of EBV DNA in culture was not concomitant with an elevation of anti-VCA IgG titers, anti-EA titers or the development of symptomatology, suggestive of a reactivation of a latent EBV infection or a progression of HIV infection, Therefore we conclude that, although interaction between both viruses may take place at the molecular level, there is no clear evidence of the repercussion that this event may have on the clinical course of HIV infection.

収録刊行物

詳細情報 詳細情報について

問題の指摘

ページトップへ