Detection of West Nile and Japanese Encephalitis Viral Genome Sequences in Cerebrospinal Fluid from Acute Encephalitis Cases in Karachi, Pakistan

  • Igarashi Akira
    Department of Virology, Institute of Tropical Medicine, Nagasaki University
  • Tanaka Mariko
    Department of Virology, Institute of Tropical Medicine, Nagasaki University
  • Morita Kouichi
    Department of Virology, Institute of Tropical Medicine, Nagasaki University
  • Takasu Toshiaki
    Department of Neurology, Nihon University Medical School
  • Ahmed Akhtar
    Department of Neurology, Dow Medical College, Civil Hospital
  • Ahmed Arsalam
    Department of Neurology, Dow Medical College, Civil Hospital
  • Akram D.S.
    Department of Pediatrics, Dow Medical College, Civil Hospital
  • Waqar M. Anwar
    Department of Biochemistry, Faculty of Human Sciences, Aga Khan University

抄録

Reverse transcriptase-polymerase chain reaction (RT-PCR) on 24 cerebrospinal fluid (CSF) specimens collected between February and August 1992 detected genome sequence of West Nile (WN) virus in 8 specimens and Japanese encephalitis (JE) virus in a single specimen. The results, combined with the data by IgM-ELISA on CSF indicated that a significant proportion of acute encephalitis cases in Karachi, Pakistan, were caused by WN virus infection, while JE virus caused a small fraction.

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