Influence of Molecular Sizes of<i>Cryptococcus neoformans</i> Capsular Polysaccharide on Phagocytosis
-
- Yasuoka Akira
- Second Department of Internal Medicine, School of Medicine, Nagasaki University
-
- Kohno Shigeru
- Second Department of Internal Medicine, School of Medicine, Nagasaki University
-
- Yamada Hiroshi
- Second Department of Internal Medicine, School of Medicine, Nagasaki University
-
- Kaku Mitsuo
- Second Department of Internal Medicine, School of Medicine, Nagasaki University
-
- Koga Hironobu
- Second Department of Internal Medicine, School of Medicine, Nagasaki University
抄録
The role of capsular polysaccharides (CPS) of Cryptococcus neoformans in phagocytosis by murine alveolar macrophages was investigated in four strains of C. neoformans serotype A, YC-11, YC-5, YC-27 and YC-13. Phagocytosis rates increased markedly after adding 10% mouse serum, compared to fetal calf serum. The reverse relation between capsular thickness of C. neoformans and phagocytosis by alveolar macrophages was observed except in YC-27, which had thin capsules and high virulence. The phagocytosis rate in mice serum was 17.3% in YC-11 (capsule thickness 2.8-3.5μm), 39.8% in YC-5 (capsule size 0.8-1.5μm), 20.3% in YC-27 (capsule size 0.6-1.1μm), and 62.8% in YC-13 (capsule not detected microscopically). The CPS of YC-11, YC-5, and YC-27 analyzed by gel-filtration using CL-2B showed high molecular fractions near the void volume. However, the CPS of YC-13 showed only low molecular fractions. The widely eluted CPS of YC-11 was separated into 3 fractions and each fraction was added in the phagocytosis assay of YC-13. Phagocytosis was markedly suppressed particularly by the addition of a higher molecular fraction. These results suggest that phagocytosis of C. neoformans by alveolar macrophages is influenced by the molecular sizes of the CPS.
収録刊行物
-
- MICROBIOLOGY and IMMUNOLOGY
-
MICROBIOLOGY and IMMUNOLOGY 38 (11), 851-856, 1994
Center For Academic Publications Japan
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1573668927872448640
-
- NII論文ID
- 130003484029
-
- ISSN
- 03855600
-
- 本文言語コード
- en
-
- データソース種別
-
- CiNii Articles