Growth Medium Containing Cyclodextrin and Low Concentration of Horse Serum for Cultivation of <i>Helicobacter pylori</i>
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- Morshed Muhammad Golam
- Department of Microbiology, Yamaguchi University School of Medicine
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- Karita Mikio
- The First Department of Internal Medicine, Yamaguchi University School of Medicine
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- Konishi Hisanori
- Department of Microbiology, Yamaguchi University School of Medicine
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- Okita Kiwamu
- The First Department of Internal Medicine, Yamaguchi University School of Medicine
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- Nakazawa Teruko
- Department of Microbiology, Yamaguchi University School of Medicine
Abstract
The growth of Helicobacter pylori, a Gram-negative microaerophilic bacterium, is often difficult and requires complex media with the supplementation of 5% to 10% blood or blood derivatives. We have found that Brucella broth supplemented with 1% heated horse serum and 0.1% β-cyclodextrin supports the good growth of H. pylori. The degree of growth and production of urease and vacuolating cytotoxin in this medium were equal to those in the medium supplemented with 5% horse serum. This medium was found to be suitable for both the routine laboratory culture and primary isolation of H. pylori from biopsy samples.
Journal
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- MICROBIOLOGY and IMMUNOLOGY
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MICROBIOLOGY and IMMUNOLOGY 38 (11), 897-900, 1994
Center For Academic Publications Japan
