Specific Detection of a Verotoxin 2 Variant, VT2vp1, by a Bead-Enzyme-Linked Immunosorbent Assay
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- Cao Chunyu
- Department of Microbiology, Faculty of Medicine, Kyoto University
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- Yamasaki Shinji
- Department of Microbiology, Faculty of Medicine, Kyoto University
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- Lin Zaw
- Department of Microbiology, Faculty of Medicine, Kyoto University
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- Kurazono Hisao
- Department of Microbiology, Faculty of Medicine, Kyoto University
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- Takeda Yoshifumi
- Department of Microbiology, Faculty of Medicine, Kyoto University
抄録
A bead-enzyme-linked immunosorbent assay to specifically detect a Verotoxin 2 variant, VT2vp1, was developed. The sensitivity of the bead-ELISA was 200pg/ml of the purified VT2vp1 and it did not react with 20ng/ml of the purified VT2. The specificity of the bead-ELISA was examined with 107 strains of Verocytotoxin-producing Escherichia coli that include VT1-, VT2-, VT2vha-, VT2vhb- and VT2vp1-producing E. coli, and only VT2vp1-producing E. coli that were confirmed by VT2vp1-specific polymerase chain reaction gave positive results. It was noted that all 58 VT2vp1-producing E. coli strains were from pigs, but not from cows and humans.
収録刊行物
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- MICROBIOLOGY and IMMUNOLOGY
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MICROBIOLOGY and IMMUNOLOGY 38 (6), 435-440, 1994
Center For Academic Publications Japan
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詳細情報 詳細情報について
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- CRID
- 1574231877825879808
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- NII論文ID
- 130003484109
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- ISSN
- 03855600
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles