Correlation of the Polysaccharide Antigens of <i>Francisella tularensis</i> with Virulence in Experimental Mice

• Fujita Hiromi

  Ohara Research Laboratory

• Sato Tadashi

  Ohara Research Laboratory

• Watanabe Yuriko

  Ohara Research Laboratory

• Ohara Yoshiro

  Department of Microbiology, Kanazawa Medical University

• Homma Morio

  Faculty of Home Economics, Kobe Women's University

抄録

Francisella tularensis gives rise to two distinct colony types, acriflavine agglutination test-positive (acf⁺) and -negative (acf^-) colonies. The acf⁺ variants were exclusively low virulent in mice, while the acf^- variants were shown to be either high or low virulent. Three fractions, phosphate-buffered saline-extractable without heating, with heating at 60C, and with heating at 100C, were obtained from cultures of both the acf⁺ and acf^- variants on agar media, and the polysaccharide antigens in those fractions were quantitated. All of the highly virulent acf^- variants possessed a large amount of the polysaccharide antigen in the fraction extractable with heating at 60C. This antigen was not, however, detected in any of the acf⁺ variants and one low-virulent acf^- variant. It was also detected in a very low amount in some other acf^- variants with low virulence. The amount of this polysaccharide antigen was therefore shown to be correlated with bacterial virulence in mice.

収録刊行物

• MICROBIOLOGY and IMMUNOLOGY

  MICROBIOLOGY and IMMUNOLOGY 39 (12), 1007-1009, 1995

  Center For Academic Publications Japan

詳細情報

• CRID: 1572824502941950720
• NII論文ID: 130003484208
• ISSN: 03855600
• Web Site: https://jlc.jst.go.jp/DN/JALC/00082149031?from=CiNii
• 本文言語コード: en
• データソース種別:
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