Characterization of an Enterotoxin Produced by <i>Vibrio cholerae</i> O139

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  • Nakashima Kazutoshi
    Department of Bacteriology, Faculty of Medicine, University of the Ryukyus
  • Eguchi Yukinori
    Laboratory Machine Center, Faculty of Medicine, University of the Ryukyus
  • Nakasone Noboru
    Department of Bacteriology, Faculty of Medicine, University of the Ryukyus|Research Center of Comprehensive Medicine, Faculty of Medicine, University of the Ryukyus

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A cholera-like enterotoxin was purified from Vibrio cholerae O139 strain AI-1841 isolated from a diarrheal patient in Bangladesh. Its characteristics were compared with that of cholera toxins (CTs) of classical strain 569B and El Tor strain KT25. AI-1841 produced as much toxin as O1 strains. The toxins were indistinguishable in terms of their migration profiles in conventional polyacrylamide gel disc electrophoresis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis and isoelectrofocusing as well as their affinity for hydroxyapatite. The skin permeability factor activity and the fluid accumulation induced in rabbit ileal loops of the toxin of AI-1841 were identical to those of the CTs. Three toxins equally reacted against anti-569B CT antiserum in Western blotting, and their B subunits formed a precipitin line against any anti-B subunit antiserum by double gel immunodiffusion. Anti-569B CTB antibody neutralized the three toxins in their PF activities and enterotoxicities. The amino acid sequence of 1841 toxin B subunit was identical with that of KT25 CTB, corresponding to the DNA sequence of ctxB from El Tor strains of the seventh pandemic. We concluded 1841 toxin was identical to CT of the seventh pandemic El Tor vibrios.

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