Correlation between the Presence of Virulence‐Associated Genes as Determined by PCR and Actual Virulence to Mice in Various Strains of <i>Listeria</i> Spp.
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- Nishibori Takeaki
- Department of Bacteriology, Niigata University School of Medicine Department of Internal Medicine (II), Niigata University School of Medicine
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- Cooray Karven
- Department of Bacteriology, Niigata University School of Medicine Department of Bacteriology, Medical Research Institute
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- Xiong Huabao
- Department of Bacteriology, Niigata University School of Medicine
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- Kawamura Ikuo
- Department of Bacteriology, Niigata University School of Medicine
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- Fujita Masashi
- Department of Bacteriology, Niigata University School of Medicine
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- Mitsuyama Masao
- Department of Bacteriology, Niigata University School of Medicine
書誌事項
- タイトル別名
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- Correlation between the Presence of Virulence-Associated Genes as Determined by PCR and Actual Virulence to Mice in Various Strains of <i>Listeria</i> Spp.
抄録
Five chromosomal genes, prfA, plcA, hlyA, mpl and plcB, are implicated in the virulence of Listeria monocytogenes and some of these genes have been used for the identification of bacteria by polymerase chain reaction (PCR). Using 6 strains of L. monocytogenes and 3 L. innocua strains, the relationship was examined between the presence of five virulence-associated genes and actual virulence to mice in terms of 50% lethal dose (LD50), bacterial viability in the organ of infected mice and the intracellular growth in cultured macrophages. None of the five genes could be amplified by PCR in all the L. innocua strains and they were actually avirulent to mice. All L. monocytogenes strains were shown to be virulent and to have intact virulence-associated genes except for the strain ATCC15313. This particular strain was revealed to be avirulent and defective in hlyA and plcA in PCR amplification. It was suggested that PCR detection of genes prfA, mpl, or plcB may not be sufficient to detect virulent strains of L. monocytogenes. It appeared that the ability to produce listeriolysin O (LLO), which is encoded by hlyA, was critical for the expression of virulence regardless of the amount of LLO produced.
収録刊行物
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- MICROBIOLOGY and IMMUNOLOGY
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MICROBIOLOGY and IMMUNOLOGY 39 (5), 343-349, 1995
財団法人 学会誌刊行センター
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キーワード
詳細情報
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- CRID
- 1390282681296697600
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- NII論文ID
- 130003484240
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- COI
- 1:CAS:528:DyaK2MXmt1aisbg%3D
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- ISSN
- 13480421
- 03855600
- http://id.crossref.org/issn/03855600
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- PubMed
- 7565175
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可