Adhesiveness for Extracellular Matrices and Lysosomal Enzyme Release from Normal and β<sub>2</sub> Integrin-Deficient Bovine Neutrophils

J-STAGE
  • Nagahata Hajime
    Department of Animal Health, Faculty of Veterinary Medicine, Rakuno Gakuen University
  • Higuchi Hidetoshi
    Department of Animal Health, Faculty of Veterinary Medicine, Rakuno Gakuen University
  • Noda Hiroshi
    Department of Animal Health, Faculty of Veterinary Medicine, Rakuno Gakuen University
  • Tamoto Koichi
    Department of Microbiology, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido
  • Kuwabara Mikinori
    Department of Environmental Veterinary Science, Graduate School of Veterinary Medicine, Hokkaido University

抄録

The adhesiveness of control and CD18-deficient bovine neutrophils on culture plates precoated with collagen I, collagen IV, fibronectin and laminin was measured to evaluate the possible factors for adherence to extracellular matrices. The release of N-acetyl-β-D-glucosaminidase (NAGase) from control and CD18-deficient neutrophils stimulated with complement receptor type 3 (CR3) or Fc receptor dependent stimuli was also evaluated. The adhesive activities of CD18-deficient neutrophils to collagen I, collagen IV and fibronectin were significantly diminished (P<0.05); however, similar adhesion to laminin was observed in CD18-deficient neutrophils and control neutrophils. The adhesive activity of control neutrophils on uncoated plates increased 2.5 times (P<0.05) with the presence of PMA. The mean activities for NAGase release from CD18-deficient neutrophils stimulated with opsonized zymosan and aggregated bovine immunoglobulin G (Agg-IgG) were 46.7 and 82.7% that of the control neutrophils, respectively. The Agg-IgG-induced NAGase release from control and CD18-deficient neutrophils was eliminated by H7, a protein kinase C inhibitor. These results support that an association between CR3 and Fc receptors on neutrophils appears to play an essential role in neutrophil functions.

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