Differential Expression of a <i>Mycobacterium tuberculosis</i> Protein Recognized by a Mouse Monoclonal Antibody
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- Cummings Patrick J.
- Department of Medical and Research Technology, University of Maryland School of Medicine
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- Rowland Sharon S.
- Department of Medical and Research Technology, University of Maryland School of Medicine
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- Hooper Nancy E.
- Department of Medical and Research Technology, University of Maryland School of Medicine
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- Schwalbe Richard S.
- Department of Medical and Research Technology, University of Maryland School of Medicine
抄録
Murine monoclonal antibodies were produced against Mycobacterium tuberculosis (Mtb) using standard hybridoma procedures. By a whole cell enzyme-linked immunosorbent assay (ELISA), one monoclonal antibody (mAb), HB28, demonstrated high level specific reactivity to Mtb. Western blot analysis demonstrated reactivity to a single 65kDa Mtb protein in the cell wall extract and culture filtrate. HB28 mAb appears to be recognizing a 65kDa Mtb protein that is over-expressed by Mtb but not other species under certain culture conditions. Differential expression and detection of this protein by HB28 mAb may have potential for diagnostic applications.
収録刊行物
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- MICROBIOLOGY and IMMUNOLOGY
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MICROBIOLOGY and IMMUNOLOGY 40 (11), 883-886, 1996
Center For Academic Publications Japan
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詳細情報 詳細情報について
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- CRID
- 1570291228151091712
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- NII論文ID
- 130003484323
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- ISSN
- 03855600
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles