Adherence Patterns and DNA Probe Types of <i>Escherichia coli</i> isolated from Diarrheal Patients in China

J-STAGE
  • Xu Jian-Guo
    Department of Microbiology, Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine
  • Cheng Bo-Qun
    Department of Microbiology, Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine
  • Wu Yan-Ping
    Department of Microbiology, Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine
  • Huang Li-Bao
    Department of Microbiology, Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine
  • Lai Xin-He
    Department of Microbiology, Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine
  • Liu Bing-Yang
    Department of Microbiology, Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine
  • Lo Xing-Zu
    Laboratory of Bacteriology, Anti-Epidemic Station of West District
  • Li Hun-Fen
    Laboratory of Bacteriology, Anti-Epidemic Station of West District

抄録

One hundred and seventy-two strains of Escherichia coli isolated from diarrheal patients in Beijing, P. R. China, were analyzed for plasmid DNA profile, HEp-2 cell adherence ability and reactivity to 10 previously described DNA probes. They had not been recognized as pathogenic E. coli in China. Of the 110 strains tested, 76 (69%) contained one or multiple large plasmids. Of the 71 strains with the large plasmids 64 could adhere to HEp-2 cells. Of the 172 strains, 102 (59.3%) were hybridized with at least one of the 10 probes. Of those, seven strains hybridized with enteroaggregative E. coli (EAggEC) probe. Their serotypes were O128 (two strains), O6 (one strain), and O111 (one strain). Three strains were untypable. Six and three strains were hybridized with enteropathogenic E. coli (EPEC) attaching and effacing genes (eae) or EPEC adherence factor (EAF) probe, respectively. Two non-O157:H7 strains hybridized with enterohemorrhagic E. coli (EHEC) probe. Seventy-two strains (41.9%) hybridized with shiga-like toxin 2 or 1 (SLT2 or SLT1) probes. Among the SLT1 or SLT2 probe-positive strains, 54 hybridized with invasive (INV) plasmid probe developed for identification of enteroinvasive E. coli (EIEC) and Shigella species. The INV and SIT probe-positive strains might represent a new variety of verotoxin-producing E. coli (VTEC).

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