Cloning and Sequencing of the Spore Germination Gene of <i>Bacillus megaterium</i> ATCC 12872: Similarities to the NaH-Antiporter Gene of <i>Enterococcus hirae</i>

• Tani Katsuji

  Department of Environmental Science and Microbiology, Faculty of Pharmaceutical Sciences, Osaka University

• Watanabe Takuya

  Department of Environmental Science and Microbiology, Faculty of Pharmaceutical Sciences, Osaka University

• Matsuda Hideyasu

  Department of Environmental Science and Microbiology, Faculty of Pharmaceutical Sciences, Osaka University

• Nasu Masao

  Department of Environmental Science and Microbiology, Faculty of Pharmaceutical Sciences, Osaka University

• Kondo Masaomi

  Department of Environmental Science and Microbiology, Faculty of Pharmaceutical Sciences, Osaka University

抄録

The germination mutant TM-31 of Bacillus megaterium ATCC 12872, was isolated by transposon Tn917 insertional mutagenesis. Glucose, L-proline, L-leucine and KNO₃ germinated TM-31 poorly. The DNA in the region of the Tn917 insertion was cloned, and its nucleotide sequence determined. One major open reading frame was present on the cloned DNA. The hydrophobic protein encoded is presumably membrane-associated. A homology search revealed that the gene encoded in the region of the Tn917 insertion is homologous to napA of Enterococcus hirae. napA codes for the NaH-antiporter. It is hypothesized that transport of cations must play an important role in spore germination in B. megaterium ATCC 12872.

収録刊行物

• MICROBIOLOGY and IMMUNOLOGY

  MICROBIOLOGY and IMMUNOLOGY 40 (2), 99-105, 1996

  Center For Academic Publications Japan

詳細情報

• CRID: 1571980078011338112
• NII論文ID: 130003484336
• ISSN: 03855600
• Web Site: https://jlc.jst.go.jp/DN/JALC/00082342814?from=CiNii
• 本文言語コード: en
• データソース種別:
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