Extraction and Characterization of the Smooth-Type Lipopolysaccharide from <i>Fusobacterium nucleatum</i> JCM 8532 and Its Biological Activities

J-STAGE
  • Onoue Sakura
    Department of Preventive and Community Dentistry, The Nippon Dental University, School of Dentistry at Tokyo
  • Niwa Moto
    Department of Preventive and Community Dentistry, The Nippon Dental University, School of Dentistry at Tokyo
  • Isshiki Yasunori
    The Kitasato Institute
  • Kawahara Kazuyoshi
    The Kitasato Institute

抄録

The lipopolysaccharide (LPS) of Fusobacterium nucleatum JCM 8532 was isolated by hot-phenol water extraction. Most of the LPS was extracted in the phenolic phase and shown to be the smooth-type, whereas the aqueous phase contained mainly rough-type LPS. The chemical composition of the LPS was similar to that reported in other studies, but D-quinovosamine, which may be a major component of O-antigenie polysaccharide, and 3-deoxy-D-manno-2-octulosonic acid (Kdo) were detected for the first time by gas chromatography-mass spectrometry. The biological activities of smooth-type LPS, including limulus activity, lethal toxicity, pyrogenicity, and B lymphocyte mitogenicity, were comparable to those of enterobacterial LPS. Smooth-type LPS inhibited the cell growth and DNA synthesis of adult and fetal human gingival fibroblasts in a dose-dependent manner, suggesting that LPS may play a role in the occurrence of human gingivitis.

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