Genetic Analysis of <i>Borrelia burgdorfe ri Sensu</i> Lato in Korea Using Genomic Hybridization and 16S rRNA Gene Sequence Determination
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- Kee Sun-Ho
- Department of Microbiology, College of Medicine, Hallym University
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- Yoon Jung-Hoon
- Korean Collection for Type Cultures, Genetic Engineering Research Institute
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- Oh Hee-Bok
- Division of Serology, National Institute of Health
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- Park Yong-Ha
- Korean Collection for Type Cultures, Genetic Engineering Research Institute
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- Kim Yoon-Won
- Department of Microbiology, College of Medicine, Hallym University
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- Cho Min-Kee
- Department of Microbiology, College of Medicine, Hallym University
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- Park Kyung-Seok
- Division of Serology, National Institute of Health
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- Chang Woo-Hyun
- Department of Microbiology, College of Medicine, Hallym University
Abstract
Nine Borrelia burgdorferi sensu lato isolated in Korea were subjected to genomic hybridization using 16S rRNA gene probe and specific restriction patterns (HindIII and EcoRV) led these nine Borrelia into five subtypes. The evolutionary relationships of the five isolates corresponding to five RFLP groups were measured through the sequence determination of 16S rRNA gene and phylogenetic analysis. The isolates 935T (group I), 934U and 17Y (Group IIa, IIb) were well clustered with B. garinii and B. afzelii. 5MT and 9MT strains (Group IIIa and Group IIIb) formed a common branch shared with B. afzelii cluster although the evolutionary distance was rather long. So, most of B. burgdorferi sensu lato in Korea was B. afzelii or B. afzelii-related group and some minor group such as B. garinii also existed.
Journal
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- Japanese Journal of Microbiology
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Japanese Journal of Microbiology 40 (9), 599-605, 1996
Center For Academic Publications Japan
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Details 詳細情報について
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- CRID
- 1570291228151619200
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- NII Article ID
- 130003484409
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- ISSN
- 03855600
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- Text Lang
- en
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- Data Source
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- CiNii Articles