Development of Functional Rat-Derived T Cells in SCID Mice Engrafted with the Fetal Thymus of LEC Rats Which Are Defective in CD4<sup>+</sup> T Cells

J-STAGE
  • Chai Jian-Guo
    Department of Parasitology and Immunology, University of Tokushima School of Medicine
  • Sakai Tohru
    Department of Parasitology and Immunology, University of Tokushima School of Medicine
  • Hisaeda Hajime
    Department of Parasitology and Immunology, University of Tokushima School of Medicine
  • Nagasawa Hideyuki
    Department of Parasitology and Immunology, University of Tokushima School of Medicine
  • Yasutomo Koji
    Department of Parasitology and Immunology, University of Tokushima School of Medicine
  • Furukawa Atuko
    Department of Parasitology and Immunology, University of Tokushima School of Medicine
  • Ishikawa Hiroyuki
    Department of Parasitology and Immunology, University of Tokushima School of Medicine
  • Maekawa Yoichi
    Department of Parasitology and Immunology, University of Tokushima School of Medicine
  • Uehara Hisanori
    Second Department of Pathology, University of Tokushima School of Medicine
  • Izumi Keisuke
    Second Department of Pathology, University of Tokushima School of Medicine
  • Matsumoto Kozo
    Institute for Animal Experimentation, University of Tokushima School of Medicine
  • Himeno Kunisuke
    Department of Parasitology and Immunology, University of Tokushima School of Medicine

抄録

We reported that LEC rats are genetically deficient in the development of thymic CD4+8- cells and that this defect is caused by bone marrow (BM)-derived stem cells. To determine which BM-derived cells are responsible for the arrest of T-cell development in LEC rats, fetal thymuses of LEC rats, or LEA rats which bear the same major histocompatibility complex (MHC) as LEC rats but are immunologically normal, were engrafted under the kidney capsule of severe combined immunodeficiency (SCID) mice (LEC- TG and LEA-TG mice, respectively). We then examined the differentiation of T cells and their immunological functions in the SCID mice. A large number of rat-derived CD4+ T cells appeared in the peripheral blood, lymph nodes (LN) and spleens in LEC-TG mice. Furthermore, the peripheral LN cells in LEC-TG mice appeared to be functional. These cells produced IL-2 upon Con A stimulation, whereas LN cells from LEC rats produced no IL-2 in the same conditions. Thymopoiesis was observed at 3 weeks in LEC-TG as well as LEA-TG mice. The distribution of thymocyte subsets with respect to CD4 and CD8 expression in LEC-TG mice closely resembled that of LEA rat thymus and that in LEA-TG mice, suggesting that normal T-cell differentiation occurred in LEC-TG mice. The results indicated that BM-derived progenitor T cells of LEC rats could differentiate to functional CD4+ T cells.

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