Participation of CD14 in the Phagocytosis of Smooth-Type <i>Salmonella typhimurium</i> by the Macrophage-Like Cell Line, J774.1
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- Onozuka Kazuyasu
- Department of Microbiology, Jichi Medical School
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- Kirikae Teruo
- Department of Microbiology, Jichi Medical School
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- Kirikae Fumiko
- Department of Microbiology, Jichi Medical School
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- Suda Yasuo
- Faculty of Science, Osaka University
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- Kusumoto Shoichi
- Faculty of Science, Osaka University
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- Yamamoto Shunsuke
- Department of Pathology, Oita Medical University
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- Shimamura Tadakatsu
- Department of Microbiology and Immunology, Showa University School of Medicine
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- Nakano Masayasu
- Department of Microbiology, Jichi Medical School
抄録
The role of CD14 in the phagocytosis and killing of microorganisms was investigated using macrophage-like cell lines, CD14-positive J774.1 cells and CD14-negative mutant J7.DEF.3 cells derived from J744.1 cells. The cells were infected with Salmonella typhimurium organisms of the smooth (S)-form LT2, mutant rough (R)-form TV148 or Staphylococcus aureus 248βH. At 30 or 180min incubation, the cells were washed and disrupted. Colony-forming units (CFUs) liberated from the disrupted cells were determined by quantitative cultivation, and the phagocytic index and killing rate were calculated. Both the phagocytic index and killing rate of J774.1 cells against LT2 organisms were greater than those of J7.DEF.3 cells. However, the index and rate of J774.1 cells against TV148 and 248βH organisms were similar to those of the J7.DEF.3 cells. The phagocytosis of LT2 organisms by J774.1 cells was partially inhibited by S-form LPS (S-LPS) and anti-CD14 antibody, but not by R-chemotype LPS (R-LPS). These results suggest that CD14 participates in the phagocytosis of S-form Salmonella.
収録刊行物
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- MICROBIOLOGY and IMMUNOLOGY
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MICROBIOLOGY and IMMUNOLOGY 41 (10), 765-772, 1997
Center For Academic Publications Japan
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詳細情報 詳細情報について
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- CRID
- 1571417128058451712
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- NII論文ID
- 130003484435
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- ISSN
- 03855600
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles