Evaluation of Enzyme-Labeled Oligonucleotide Probes to Identify Enterohaemorrhagic <i>Escherichia coli</i>

• Yoh Myonsun

  Research Center for Emerging Infectious Diseases, Research Institute for Microbial Diseases, Osaka University

• Takagi Kazuhiro

  Quarantine Station of Osaka

• Eda Junji

  Quarantine Station of Osaka

• Ohtomo Masato

  Quarantine Station of Osaka

• Takarada Yutaka

  Toyobo Research Center

• Shibata Shuji

  Toyobo Research Center

• Honda Takeshi

  Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University

抄録

Alkaline phosphatase-conjugated oligonucleotide probes were developed to detect the gene coding for Vero toxin 1 (VT1) and Vero toxin 2 (VT2). Using these probes, 3hr was enough to detect VT genes when suspicious colonies of enterohaemorrhagic Escherichia coli (EHEC) were obtained on an agar plate. The results of a hybridization test with 144 isolates of EHEC O157 and one isolate of Shigella dysenteriae Type 1 agreed exactly with the immunological detection, reversed passive latex agglutination (RPLA) test, of VTs in their culture supernatants. The sensitivity levels of these probes for the detection of VT genes were 100%. The specificity of these probes were also tested with a total of 1, 002 strains of Escherichia coli other than EHEC and 8 strains of Shigella sp. other than Shigella dysenteriae Type 1; the results showed 100% specificity.

収録刊行物

• MICROBIOLOGY and IMMUNOLOGY

  MICROBIOLOGY and IMMUNOLOGY 41 (11), 879-882, 1997

  Center For Academic Publications Japan

詳細情報

• CRID: 1571417128058465152
• NII論文ID: 130003484455
• ISSN: 03855600
• Web Site: https://jlc.jst.go.jp/DN/JALC/00084331267?from=CiNii
• 本文言語コード: en
• データソース種別:
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