Application of Equine Infectious Anemia Virus Core Proteins Produced in a Baculovirus Expression System to Serological Diagnosis

• Kong Xian-Gang

  National Institute of Animal Health

• Pang Hai

  National Institute of Animal Health

• Sugiura Takeo

  Epizootic Research Station, Equine ResearchInstitute, Japan Racing Association

• Sentsui Hiroshi

  National Institute of Animal Health

• Onodera Takashi

  Department of Molecular Immunology, School of Agriculture and Life Sciences, the University of Tokyo

• Matsumoto Yoshitsugu

  Department of Molecular Immunology, School of Agriculture and Life Sciences, the University of Tokyo

• Akashi Hiroomi

  National Institute of Animal Health

Abstract

Equine infectious anemia virus (EIAV) core proteins were obtained from a baculovirus expression system. Recombinant baculoviruses (rBVs) highly expressed the Gag precursor and p26 antigens in an rBV-infected Sf21 cell culture supernatant. Enzyme-linked immunosorbent assay (ELISA) and agar gel immunodiffusion (AGID) were conducted using the expressed proteins to detect antibodies from experimentally infected horses. The expressed antigens showed low background levels, high specificity and sensitivity in ELISA and AGID. The results of the serological tests using the expressed antigens were identical to those using a manufactured trial antigen. rBVs containing gag and p26 genes were found to express high quality and large quantities of Gag and p26 antigens, respectively. The antigens were quite useful for detecting anti-EIAV antibodies from virus-infected horses

Journal

• Japanese Journal of Microbiology

  Japanese Journal of Microbiology 41 (12), 975-980, 1997

  Center For Academic Publications Japan

Details

• CRID: 1571698603035179264
• NII Article ID: 130003484468
• ISSN: 03855600
• Web Site: https://jlc.jst.go.jp/DN/JALC/00084452825?from=CiNii
• Text Lang: en
• Data Source:
  • CiNii Articles