Pathogenicity of Glycoprotein C-Deficient Herpes Simplex Virus 1 Strain TN-1 Which Encodes Truncated Glycoprotein C
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- Minagawa Hiroko
- Department of Virology, Faculty of Medicine, Kyushu University
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- Liu Ying
- Department of Virology, Faculty of Medicine, Kyushu University
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- Yoshida Tetsuhiko
- Department of Virology, Faculty of Medicine, Kyushu University
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- Hidaka Yasufumi
- Department of Virology, Faculty of Medicine, Kyushu University
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- Toh Yasushi
- Department of Virology, Faculty of Medicine, Kyushu University
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- Mori Ryoichi
- Department of Virology, Faculty of Medicine, Kyushu University
抄録
A clinical isolate of herpes simplex virus 1 (TN-1) from a stromal keratitis patient was found to be defective in the glycoprotein C (gC) gene (UL44), thus resulting in the production of truncated gC upon infection. To study the pathogenetic role of truncated gC, we prepared a recombinant LTN-8 derived from TN-1 with deletions of the 1.5 kilobase pairs of the gC gene including the initiation codon. A penetration assay revealed LTN-8 to be less efficient in its penetration ability than TN-1, the laboratory strain KOS and RTN-1-20-3, a recombinant derived from TN-1 with the KOS gC gene. The penetration of LTN-8 was facilitated by the addition of TN-1-infected culture medium. TN-1 virus preparations had no hemagglutinating activity. However, the animals infected with TN-1 did develop hemagglutination inhibition (HI) antibodies. The LTN-8-infected animals did not develop HI antibodies. The pathogenicity in BALB/c mice following either corneal, intraperitoneal or intracerebral inoculation did not significantly differ among TN-1, RTN-1-20-3 or LTN-8. Our results indicate that truncated gC was sufficient for the induction of HI antibodies and was also able to facilitate penetration in vitro. Although truncated gC might be a virulence factor acting as a decoy, both truncated gC and intact gC had little effect on the outcome following intracerebral, intraperitoneal or corneal inoculation.
収録刊行物
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- MICROBIOLOGY and IMMUNOLOGY
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MICROBIOLOGY and IMMUNOLOGY 41 (7), 545-551, 1997
Center For Academic Publications Japan
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詳細情報 詳細情報について
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- CRID
- 1572261552988311552
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- NII論文ID
- 130003484536
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- ISSN
- 03855600
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles