Combined Use of Ribotyping, PFGE Typing and IS<i>431</i> Typing in the Discrimination of Nosocomial Strains of Methicillin-Resistant <i>Staphylococcus aureus</i>
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- Yoshida Tatsumi
- Department of Bacteriology, Juntendo University
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- Kondo Noriko
- Department of Bacteriology, Juntendo University
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- Hanifah Yasmin Abu
- Department of Clinical Microbiology, University of Malaya
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- Hiramatsu Keiichi
- Department of Bacteriology, Juntendo University
抄録
We have previously reported the phenotypic characterization of methicillin-resistant Staphylococcus aureus (MRSA) clinical strains isolated in Malaya University Hospital in the period 1987 to 1989 using antibiogram, coagulase typing, plasmid profiles, and phage typing. Here, we report the analysis of the same strains with three genotyping methods; ribotyping, pulsed-field gel electrophoresis (PFGE) typing, and IS431 typing (a restriction enzyme fragment length polymorphism analysis using an IS431 probe). Ribotyping could discriminate 46 clinical MRSA strains into 5 ribotypes, PFGE typing into 22 types, and IS431 typing into 15 types. Since the differences of the three genotyping patterns from strain to strain were quite independent from one another, the combined use of the three genotyping methods could discriminate 46 strains into 39 genotypes. Thus, the powerful discriminatory ability of the combination was demonstrated.
収録刊行物
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- MICROBIOLOGY and IMMUNOLOGY
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MICROBIOLOGY and IMMUNOLOGY 41 (9), 687-695, 1997
Center For Academic Publications Japan
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詳細情報 詳細情報について
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- CRID
- 1570854178104763648
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- NII論文ID
- 130003484547
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- ISSN
- 03855600
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles