Tinctorial Properties of Spherical Bodies in Broth Cultures of <i>Nocardia asteroides</i> GUH-2

• Kohbata Shunro

  Department of Microbiology, Gifu University School of Medicine

抄録

In yeast extract-supplemented brain heart infusion (BHI) broth cultures of Nocardia asteroides GUH-2, many spherical bodies (SBs) were frequently seen nearby filamentous cells. They showed no Gram-positivity when Gram stain was applied. When acridine orange stain was applied, many of them showed different green fluorescence from bright orange fluorescence of the filamentous nocardiae under ultraviolet light. Their acid-fastness appeared to depend on the presence of paraffin. Using the polymerase chain reaction (PCR) method, 16S rRNA genes were detected in SB-containing broth cultures inoculated with culture filtrates from broth cultures of the strain and identical to that of N. asteroides. These results suggest that SBs are cell wall-defective (CWD) forms which result from the spontaneous mutation of N. asteroides GUH-2.

収録刊行物

• MICROBIOLOGY and IMMUNOLOGY

  MICROBIOLOGY and IMMUNOLOGY 42 (3), 151-157, 1998

  Center For Academic Publications Japan

詳細情報

• CRID: 1571980078011603584
• NII論文ID: 130003484577
• ISSN: 03855600
• Web Site: https://jlc.jst.go.jp/DN/JALC/00084687371?from=CiNii
• 本文言語コード: en
• データソース種別:
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