Antigenic and Functional Analyses of Glycoprotein of Rabies Virus Using Monoclonal Antibodies
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- Luo Ting Rong
- Department of Veterinary Public Health, Faculty of Agriculture, Gifu University
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- Minamoto Nobuyuki
- Department of Veterinary Public Health, Faculty of Agriculture, Gifu University
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- Hishida Miyuki
- Department of Veterinary Public Health, Faculty of Agriculture, Gifu University
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- Yamamoto Keiko
- Department of Veterinary Public Health, Faculty of Agriculture, Gifu University
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- Fujise Toru
- Department of Veterinary Public Health, Faculty of Agriculture, Gifu University
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- Hiraga Shinya
- Department of Veterinary Public Health, Faculty of Agriculture, Gifu University
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- Ito Naoto
- Department of Veterinary Public Health, Faculty of Agriculture, Gifu University
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- Sugiyama Makoto
- Department of Veterinary Public Health, Faculty of Agriculture, Gifu University
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- Kinjo Toshio
- Department of Veterinary Public Health, Faculty of Agriculture, Gifu University
抄録
Thirty-five monoclonal antibodies (MAbs) against glycoprotein (G protein) of the RC-HL strain of the rabies virus have been established. Using these MAbs, two antigenic sites (I and II) were delineated on the G protein of the RC-HL strain in a competitive binding assay. Of these, 34 MAbs recognized the epitopes on site II. Site II was further categorized into 10 subsites according to their patterns in a competitive binding assay. Each site II-specific MAb showed 5 to 23 nonreciprocal competitions. The reactivities of 35 MAbs to rabies and rabies-related viruses in an indirect immunofluorescent antibody test showed that six MAbs in group A binded to rabies and rabies-related viruses and eight MAbs in group E reacted only with rabies viruses, considering that the former represent the genus-specific of Lyssavirus and the latter are rabies virus-specific. From biological assays, 28 of the 35 MAbs showed neutralization activity, 31 showed hemagglutination inhibition (HI) activity, and 18 showed immunolysis (IL) activity. The MAbs recognizing neutralization epitopes fell into at least three groups: those exhibiting both HI and IL activity, those showing only HI activity, and those showing neither HI nor IL activity. All IL epitopes overlap with HA epitopes. Five of the nine MAbs which reacted with the antigen treated by sodium dodecyl sulfate in ELISA were not reduced, or reduced only slightly, in the titer. None of the MAbs reacted with 2-mercaptoethanol-treated antigen. Only one MAb that recognized site I reacted with the denatured G protein in a Western blotting assay, indicating that its epitope is linear. These results suggest that almost all of the epitopes on the G protein of the rabies virus are conformation-dependent and the G protein forms a complicated antigenic structure.
収録刊行物
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- MICROBIOLOGY and IMMUNOLOGY
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MICROBIOLOGY and IMMUNOLOGY 42 (3), 187-193, 1998
Center For Academic Publications Japan
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詳細情報 詳細情報について
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- CRID
- 1570572703128057344
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- NII論文ID
- 130003484582
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- ISSN
- 03855600
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles